Polymer Chemistry

Polyglycidol of hyperbranched architecture (HbPGL) is able to interact with boronic acids thanks to vicinal diol groups present in the peripheral area of the macromolecule. As a result, injectable hydrogel systems can be formed at different pH conditions. Bioinert and protein antifouling characteristics of HbPGL makes hydrogels prepared from this polymer a suitable system for biomedical applications. Hydrogel environment turned out to be completely inert to alkaline phosphatase, which facilitated triggering the biomineralization Due to the fact that diffusion of reagents limits the rate of enzymatic reactions within gel, the influence of polymer weigh fraction and the length of cross-linking copolymer bearing boronic acid on the diffusivity of encapsulated in hydrogel molecules was estimated. The dynamics of macromolecules forming hydrogel network was monitored based on their diffusion coefficients using 1H HR MAS DOSY NMR. Depending on the diffusivity, the course of biomineralization within hydrogel was different. 1H NMR DOSY NMR spectra, along with standard 1H NMR spectra, were also used to monitor the behaviour in solution of another type cross-link, which is a homodimer of molecular clips. By making use of this affinity, we can prolong the macromolecule of the polymer or generate the polymer network. The affinity toward homodimerization is strictly dependent on the solvent, temperature and on the presence of other compounds, which can play the role of the guest molecules. Due to the fact that homodimerization is driven both by π-π interactions and hydrogen bonds formation, such solvents as DMSO prevents dimers formation, whereas for example chloroform stimulates the selfassociation of molecular clips.